(with video tracking using EthoVision)
Delivery of therapeutic agents, lenti-viruses, and most recently DREADDs through intracerebro-ventricular (ICV) or intrahippocampal injections
Implantation of microelectrode arrays to measure the kinetics (i.e., release and uptake) of various neurotransmitters, such as glutamate. Implantation of fiber optic cannula for use in optogenetic studies.
In Vivo Glutamate Recordings
(amperometry/chonoamperometry system from Quanteon)
Recording of potassium chloride (KCl) evoked glutamate release in the hippocampus of a mouse
We insert light-sensitive receptor proteins into single neurons in vivo, making these neurons sensitive to activation by light of specific wavelengths. This allows us to control the activity of these neurons and study their downstream influence on behavior.
Neuron loss in the hippocampus of a mouse model of Alzheimer's
Transgenic Mouse Models of Alzheimer's Disease
Using hippocampal neurons transfected with GFP-tagged tau, we examine the aberrant localization of tau to dendritic spines.