Gentaur® 2X Phanta Max Master Mix - 1mL
2× Phanta Max Master Mix is a ready-to-use high-fidelity PCR reagent optimized for fast and accurate DNA amplification. It includes Phanta Max DNA Polymerase, dNTPs, and a reaction buffer that supports robust performance across GC-rich, long-template, and complex DNA samples. This mix is suitable for standard PCR, high-fidelity amplification, cloning, genotyping, and mutagenesis.
✅ Key Features
- High Fidelity: Up to 50× higher accuracy than conventional Taq polymerase
- Speed: Amplifies 1–10 kb DNA fragments in seconds per kilobase
- Robust Performance: Reliable results on GC-rich and long templates
- Simple Workflow: Just add primers and DNA templates
- Direct Gel Loading: Contains tracking dyes for direct electrophoresis
- Wide Compatibility: Ideal for plasmid, genomic DNA, and cDNA templates
🔬 Applications
- Routine PCR amplification
- Gene cloning
- Site-directed mutagenesis
- Colony PCR
- High-throughput genotyping
- Next-generation sequencing (NGS) preparation
🧪 Technical Details
- Polymerase Type: High-fidelity DNA polymerase with proofreading activity
- Extension Rate: 15–30 seconds/kb under optimized conditions
- Fragment Length: Efficient for amplicons up to 20 kb (genomic)
- Storage: Stable at -20°C, with anti-freezing buffer
- Concentration: 2× premixed format
🧫 Performance Data
In benchmarking tests, Phanta Max Master Mix demonstrates equal or better performance than standard high-fidelity enzymes. The product maintains high specificity and low background noise, even with difficult templates.
📦 Packaging Options
- 1 mL (for ~100 reactions)
- 5 × 1 mL
- 15 × 1 mL
- Custom bulk options available